Replication for: Single torradovirus infections explain the mysterious cassava frogskin disease in the Americas

Sentinel cassava plants were exposed to high disease pressure in the field to promote root symptom expression. After visual inspection, symptomatic plants were propagated in a screenhouse for a second growth cycle. Molecular analyses, including RT-PCR and HTS, were conducted to identify associated pathogens and investigate their link to cassava frogskin symptoms.

Methodology: Sentinel plants were exposed to high disease pressure for one crop cycle to allow the development of root symptoms. At the end of the exposure period, a visual assessment was conducted to detect cassava frogskin symptoms. Selected plants were propagated and maintained under controlled conditions in a screenhouse for a second growth cycle, allowing the development of storage roots. Collected samples were subjected to molecular analyses, including targeted RT-PCR and HTS, to identify the presence of different pathogens and perform association studies with the observed symptoms.